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నైరూప్య

The Diminished Ovarian Reserve with Chronic Stress Induced by Tripterygium with Chronic Unpredictable Mild Stress in SD Rats

Yanhua Li1#, Dongqin Xia1*, Wei Wang1*, Jun Liu2

Aim: To establish a rat model of Diminished Ovarian Reserve (DOR) combined with Chronic Unpredictable Mild Stress (CUMS) and to explore the effect of chronic adverse psychological stress on ovarian reserve dysfunction, so as to provide experimental basis for further research.

Methods: The rats were randomly divided into control group, CUMS group, DOR group and DOR+CUMS group, with 10 rats in each group, given fed normally, chronic mild unpredictable stress, tripterygium glycoside tablets and tripterygium glycoside tablets with CUMS intervention in proper order for 21 days. We observed the body mass, estrous cycle, behavioral testing, the Hematoxylin and Eosin (H&E) staining of ovarian tissue, the serum levels (E2, FSH, 5-HT and GnRH) and Apoptotic granulosa cells of ovarian tissue.

Results: After a 21-days exposure, in DOR group or DOR+CUMS group the serum levels of E2 was reduced and the FSH was raised. In DOR group, the estrous cycle was disordered, the ovary was slightly atrophied and the ovarian tissue structure was not clear, the number of follicles and the content of follicular fluid were small, the cell layers was reduced, and inflammatory cell infiltration was visible, the change was more obvious in DOR+CUMS group. In CUMS group and DOR+CUMS group, the OFT, SPT and 5-HT were lower and the GnRH was higher than in control group. About the TUNEL, the AI in the DOR group, the CUMS group and DOR+CUMS group were higher.

Conclusion: Our outcomes state that we could establish a rat model of psychological stress-induced DOR successfully which can be used for further study.