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నైరూప్య

Evaluation of Cytoprotective and In vivo Anti-Oxidant Activity of Argyreia Nervosa Leaves Extract against Hydroquinone Induced Genotoxicity

Divya O, Pushpakumari B, Anil Kumar D, Nihafiaz S, Sameer P

Hydroquinone (HQ) is a major benzene metabolite, which is produced after benzene biotransformation mainly in liver, lungs and bone marrow. However, the effects of mechanisms, in particular related to its genotoxicity in humans are not well understood. Argyreia nervosa (AN) is traditionally used in rheumatic, cerebral disorders, syphilis, and wound healing as immunomodulatory agent. The aim of this study was to access the protective effect of quercetin present in hydroalcoholic extract of Argyreia nervosa leaves against HQ-induced genotoxicity in rats and to identify and clarify the mechanisms, using albino rats of wistar strain bone marrow cells. The data suggested that HQ caused DNA strand breaks, DNA Protein Crosslink’s (DPC) and chromosomal breaks and significant increase in the frequency of micronuclei was found in the Micronucleus Test (MNT) and chromosomal aberrations assay. To elucidate the Oxidative DNA damage mechanism, parameters such as antioxidant enzymes like LPO, SOD, GSH and catalase levels were chosen to monitor the levels of Reactive Oxygen Species (ROS) and Glutathione (GSH) respectively. The present study showed that HQ induced the increased levels of ROS and depletion of GSH in rats; quercetin exerted an antigen toxic effect on HQ- induced genotoxicity, by significantly reducing the number of structural aberrations in chromosomes. Our results demonstrate that quercetin exerts a cytoprotective effect against HQ-induced oxidative stress, DNA damage and genotoxicity.