బయోమెడికల్ ఇంజనీరింగ్ మరియు మెడికల్ డివైజెస్ జర్నల్
అందరికి ప్రవేశం
ISSN: 2475-7586
ISSN: 2475-7586
Zahra Majedi, Armaghan Lohrasbi, Marjan Rahbar, Shadi Hajrasouliha, Sepideh Khaleghi*
Background: The wound healing process comprises of highly complex and dynamic events which allow the reestablishment of skin's structural integrity. To overcome the drawbacks associated with this process, researchers have been focused on the development of new therapeutics. To meet this demand, an interesting option is Chitosan based biopolymers. This study develops and evaluates a combination of Chitosan and bioactive Spirulina microalgae, extract hydrogel as skin serum to enhance the wound healing process.
Methods: Crude extract of dry S. platensis was prepared using three solvents (methanol, ethanol and water). Spirulina Extract-Chitosan Hydrogels (SECH) was synthesized using an ionic dilution method. SECH was characterized using Dynamic Light Scattering (DLS) and TEM microscopy. In vitro cytotoxicity of nano hydrogels on HFF-1 fibroblast Skin Normal cells was determined using MTT assay. Scratch assay was performed to evaluate the wound healing properties of SECH. QReal Time PCR was applied to evaluate the effect of SECH on gene expression of TNF-ß and PDGF.
Results: Aqueous SECH (SECH-W) showed higher proliferation activity compared with the control group by 1.78- fold and 2.55-fold increase of cell viability after 24 and 48 hr respectively with 3 mg/ml concentration. Aqueous SECH enhanced wound healing process on HFF-1 cell line with 100% wound closure after 48 hrs. TNF-ß and PDGF gene expressions were up-regulated by 3-fold and 4.2-fold after 24 h incubation, respectively.
Conclusions: Our findings showed that the SECH-W increased the proliferation of HFF-1 cells significantly and promotes the wound healing process in vitro. Therefore, the Spirulina-chitosan hydrogel could be of potential value in cosmeceutical and biomedical applications.