జర్నల్ ఆఫ్ క్రోమాటోగ్రఫీ & సెపరేషన్ టెక్నిక్స్
అందరికి ప్రవేశం

నైరూప్య

Validated UPLC Method for Identification and Quantification of Phenazopyridine Hydrochloride Drug: A Green Analytical Technique

Omprakash Saini*, Sanjay Kumar Sharma, Yogesh Sharma

Green Chemistry talks about any environmental technique or practice which may reduce waste, materials, hazards, risk, energy, environmental impact, and operational cost, also increase productivity. Ultra Performance Liquid Chromatography (UPLC) is a modernized technique of High-performance Liquid chromatography (HPLC), which is a Green Technique in the true sense. To enhance those practices, a simple, sensitive, accurate, selective, and precise Ultra Performance Liquid Chromatography (UPLC) method was developed for the identification and quantification of the Phenazopyridine Hydrochloride Drug. The separation was performed on a BEH C18-column (1.7 µm, 2.1 mm × 50 mm) using a mobile phase 20 mM ammonium acetate buffer as mobile phase A and 100% Acetonitrile as a mobile phase-B in gradient elution. The detection was performed at a fixed wavelength (λ=280 nm), with a flow rate of 0.40 ml/min. The retention time of Phenazopyridine hydrochloride was found at about 4.67 minutes. After developing the method, it was assured for future use by validation of the analytical parameters like System Suitability, Specificity, linearity, accuracy, precision, solution stability, and robustness. The results of all the parameters of the method were found within the acceptance criteria as per the International Council for Harmonization (ICH) guidelines and general practices of pharmaceutical industries.